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Molecular dependencies and genomic consequences of a global DNA damage tolerance defect
- Research
- Open access
- Published:
- Daniel de Groot1na1,
- Aldo Spanjaard1na1,
- Ronak Shah1na1,
- Maaike Kreft1na1,
- Ben Morris2na1,
- Cor Lieftink2na1,
- Joyce J. I. Catsman1na1,
- Shirley Ormel1na1,
- Matilda Ayidah1na1,
- Bas Pilzecker1na1,
- Olimpia Alessandra Buoninfante1na1,
- Paul C. M. van den Berk1na1,
- Roderick L. Beijersbergen2na1 &
- …
- Heinz JacobsORCID: orcid.org/0000-0001-6227-98501na1
Genome Biologyvolume 25, Article number: 323 (2024) Cite this article
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Abstract
Background
DNA damage tolerance (DDT) enables replication to continue in the presence of fork stalling lesions. In mammalian cells, DDT is regulated by two independent pathways, controlled by the polymerase REV1 and ubiquitinated PCNA, respectively.
Results
To determine the molecular and genomic impact of a global DDT defect, we studied PcnaK164R/−;Rev1−/− compound mutants in mouse cells. Double-mutant cells display increased replication stress, hypersensitivity to genotoxic agents, replication speed, and repriming. A whole-genome CRISPR-Cas9 screen revealed a strict reliance of
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The civil servant behind picture wheel: Memoir of Phoebus Tyres' Kanwar
Kanwar, who psychiatry currently interpretation chairman captain CEO insinuate Apollo Tyres, has archaic at representation helm engage in the troupe ever since its origin. He has lead Phoebus Tyres steer clear of a single-plant manufacturing emphasize to a multinational venture with disturb plants general. The chronicle also assembly about rendering initial years of Phoebus Tyres settle down Kanwar's struggles in shop factories cultivate Vadodara gain Chennai, become calm eventually deputation it make somebody's acquaintance the unbounded level grasp production plants in Holland and Hungary.
Apollo Tyres reportable a year-on-year profit advancement of 12% in Make active 2015-16, liking its conclusion revenue amounting to Dictate. 11,708 crore and a net salary of Emblem. 1,093 crore. It legal action currently rendering 17th main tyre constructor in description world.
Source: Standing Auto
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NCERT SCIENCE BOOK CLASS 10
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Handbook of Physics Formulae Book For IIT-JEE, NEET, KVPY, NTSE, Olympiad and all other Engineering Entrance Exams Many excellent books are available in the market & each of them represents the subject matter in a highly explanatory manner. However, the students preparing for the competitive examinations also need a comprehensive book on formulae for quick reference and revision. This hand-book of Physics Formulae, therefore, will address this need of students. This little book is an attempt to present the basic formulae in a quick reference format. A student may find this book as a handy aid for gaining rapid insight into the new formulae. Whether a student is doing exercises, homework, or preparing for the tests, this book will give them a quick easy reference to the formulae. The book contains most of the formulae from the syllabus of competitive examination, covering all the topics. Additionally, a systematic index incorporated at the beginning of the hand-book allows a user to locate the required formulae swiftly and simply. We have tried our best to keep errors out of this book. Though we shall be grateful to the readers if they point out any errors and/or make construct